The EMXtalOrg suite was written to provide a user-friendly interface to
the electron microscopy (EM) 3-D merging program origtilt, which was
written in user-unfriendly Fortran. It also serves to provide functions in the processing
of crystalline EM image data after indexing and correction for the
contrast transfer (CTF) effect. The current version uses
amplitudes-and-phases (aph) data files from indexed, tilted EM images of
two-dimensional (2D) protein crystals, such as those output by the SPECTRA
indexing program included with the Integrated Crystallographic Environment
(I.C.E.) suite (
http://ncmi.bcm.tmc.edu/software/software.html). After
merging the files in tilt_org and processing with aph_edit, a standard
HKL-type file or several program-specific file-types can be output which
can be used as input to a number of 3-D image processing and visualization
programs including CCP4 (http://
www.dl.ac.uk/CCP/CCP4/main.html) and XtalView
(http://www.scripps.edu/pub/dem-web/).